| 质粒名称: | Anti-6xHis [N144/14R] |
|---|---|
| 目录编号: | 177500 |
| 存储实验室: | James Trimmer |
|
载体骨架基本信息 |
|
| 载体名称: | P1316-IgG2a |
| 载体出品公司: | Gavin Wright, Sanger; Yves Durocher, NRC |
| 空载体大小: | 5025 bp |
| 完整质粒大小: | 9403 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | anti-6xHis recombinant mouse monoclonal antibody |
| 物种来源: | M. musculus (mouse) |
| 启动子: | Dual CMV |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | Anti-6xHis [N144/14R] was a gift from James Trimmer (Addgene plasmid # 177500 ; http://n2t.net/addgene:177500 ; RRID:Addgene_177500) |
| 参考文献部分: | High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research. Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles ML, Froenicke L, Trimmer JS. Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4. 10.1038/s41598-023-43233-4 PubMed 37758 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
